NECi Monoclonal Antibodies for Nitrate Reductase

3-D Model of an antibody showing black regions where antigen combines to form a tight complex.

NECi Antibodies for Plant Nitrate Reductase
(Anti-NaR):

Antibodies for Squash and Corn Nitrate Reductase (NaR) are produced in 2 forms using purified Squash (Cucurbita maxima) and Corn (Zea mays) NADH: NaR - rabbit polyclonals and murine monoclonals.

Both types of antibodies are purified to yield the Immunoglobulin fraction (IgG) using Protein-A Sepharose affinity chromatography. The rabbit polyclonal anti-NaR are useful for Western Blotting and ELISA immunochemical methods [1], while the monoclonal anti-NaR are most useful for the immunoaffinity chromatographic purification of NaR forms [2].

Anti-NaR are shipped either as a freeze-dried powder, which is reconstituted with deionized water, or in a phosphate at pH 7.

Datasheets with Prices for NECi Anti-NaR:

Corn Monoclonal Anti-NaR
Corn Polyclonal Anti-NaR
Squash Monoclonal Anti-NaR
Squash Polyclonal Anti-NaR (no longer available)

As shown on the datasheets, all these Anti-NaR are available as the free IgG in solution, or with the IgG immobilized on Sepharose.

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References (copies of these papers are available by request):

[1] Campbell, W. H., and J. L. Remmler (1986) Regulation of corn leaf nitrate reductase: I. Immunochemical methods for analysis of the enzyme's protein component. Plant Physiology 80: 435-441.

[2] Hyde, G. E., J. A. Wilberding, A. L. Meyer, E. R. Campbell, and W. H. Campbell (1989) Monoclonal antibody-based immunoaffinity chromatography for purifying corn and squash NADH: nitrate reductase. Plant Molecular Biology 13: 233-246.