NECi Antibodies for Plant Nitrate Reductase
(Anti-NaR)

3-D Model of an antibody showing black regions
where antigen combines to form a tight complex.
Revised and Updated: 01/21/2010                                                                   1.888.NITRATE  www.nitrate.com
Antibodies for Squash and Corn Nitrate Reductase (NaR) are produced in 2 forms using purified Squash (Cucurbita maxima) and Corn (Zea mays) NADH: NaR - rabbit polyclonals and murine monoclonals. 

Both types of antibodies are purified to yield the Immunoglobulin fraction (IgG) using Protein-A Sepharose affinity chromatography. The rabbit polyclonal anti-NaR are useful for Western Blotting and ELISA immunochemical methods [1], while the monoclonal anti-NaR are most useful for the immunoaffinity chromatographic purification of NaR forms [2].

Anti-NaR are shipped freeze-dried, and is reconstituted with deionized water or in a phosphate buffer at pH 7.
Datasheets with Prices for NECi Anti-NaR:

As shown on the datasheets, all these Anti-NaR are available as the free IgG in solution, or with the IgG immobilized on Sepharose.

To view these datasheets download Adobe Acrobat PDF Reader

 
References (copies of these papers are available by request)

 

 [1] Campbell, W. H., and J. L. Remmler (1986) Regulation of corn leaf nitrate reductase: I.  Immunochemical methods for analysis of the enzyme's protein component.  Plant Physiology 80: 435-441.

[2] Hyde, G. E., J. A. Wilberding, A. L. Meyer, E. R. Campbell, and W. H. Campbell (1989) Monoclonal antibody-based immunoaffinity chromatography for purifying corn and squash NADH: nitrate reductase. Plant Molecular Biology 13: 233-246.