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Bio-Medical Nitrate Assays
Nitrate Reductase Based!
Superior
Stock Nitrate Reductase Inside!
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Nitric Oxide (NO) Produced in Body Tissues and Fluids Results
in Residual
Nitrate in the Human Body
NO produced in response to infection and stress raises the Nitrate level over that
normally found in the serum. This is due to conversion of NO to nitrate and nitrite.
Low levels of nitrate in serum are normal due to hormonal NO production and dietary
intake. However, when the body is being challenged, the NO is often produced and
results in an elevated level of nitrate, which can be viewed as an indicator of increased
production of NO.
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| In research studies following NO levels, the transiently produced
NO gas is difficult to detect unless expensive instrumentation is available. Since
NO is stoichiometrically converted to nitrate and nitrite, the levels of the NO produced
in a response can be tracked by assaying for nitrate and nitrite. The easiest test
for nitrate is done by converting it to nitrite by enzymatic reduction catalyzed by
nitrate reductase. The nitrite is detected by a simple colorimetric assay using the
Griess Reagents. This approach works well in tissue culture based studies and
isolated body tissues and fluids. |
| NECi Lab Nitrate Test Kits (Lab-NTK) use small volumes of sample
(5 to 500 µL) and generally have no problem with interferences due to protein content of
the samples. Micro Plate Format is available for investigators with Micro Plate
Readers. Test Tube Format is available for investigators with a colorimeter or
spectrophotometer. NADH: Nitrate Reductase is used in NECi Lab-NTK, the reagents are
less costly and the kits cheaper. Lab-NTK are highly sensitive, reproducible and
accurate. To improve linearity of the Nitrate Standard Curve, a quench agent is
provided to remove excess NADH from low nitrate sample.
BioMedical Literature on Enzyme-Based Nitrate Assays for NO Research:
- Granger DL, RR Taintor, KS Broockvar & JB Hibbs (1996) Measurement of
nitrate and nitrite in biological samples using nitrate reductase and Griess reaction.
Methods in Enzymology 268: 142-151
- Grisham, MB, GG Johnson, & JR Lancaster (1996) Quantitation of
nitrate and nitrite in extracellular fluids. Meth. Enzymol. 268: 237-245
- Guevara, I, J Iwanejko, A Szczudlik (1998) Determination of
nitrite/nitrate in human biological material by the simple Griess reaction. Clin Chim Acta
274:177-88.
- Rao, AM, A Dogan, JF Hatcher, RJ Dempsey (1998) Fluorometric assy of
nitrite and nitrate in brain tissue after traumatic brain injury and cerebral ischemia.
Brain Res 793:265-270. Some features of this method are incorporated in NECi
Micro Plate Nitrate Test Kits.
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NECi Lab Nitrate Test Kits
- Based on the Enzyme
Superior Stock Nitrate Reductase
- Small Sample Volume: 5 to 500 µL
- 2 Formats: Micro Plate and Test Tube
- 2 Nitrate Ranges: Low = 3.6 to 71.4 µM &
Std = 36 to 714 µM
Potential Applications:
- Nitric Oxide (NO) Research
- Organ Transplant Studies
- Sepsis Indicator
- Low Grade Infections
- Inflammation Indicator
No Sample Preparation for:
- Tissue Culture Media
- Blood Plasma
- Serum
- Urine
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NECi Lab Nitrate Test Kits (NTK)
Call 1-888-NITRATE
(648-7283) to Order
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NECi Lab Nitrate Test Kits (L-NTK)
Less expensive than most other commercial NO/Nitrate Test Kits
Micro Plate Nitrate Test Kits
96 Well Micro Plate Format
You need a Micro Plate Reader!
- Low Range (3.6 to 71.4 µM Nitrate):
Catalog No. M-NTK-302 - $125 plus shipping ($5 for USA Addresses)
Sample Size = 50 µl or 0.05 ml -- 96 Well Micro Plate provided
- Standard Range (35.7 to 714 µM Nitrate):
Catalog No. M-NTK-301 - $125 plus shipping ($5 for USA Addresses)
Sample Size = 5 µl or 0.005 ml -- 96 Well Micro Plate provided
Test Tube Format -- Colorimeter Required
The Only Test Tube Nitrate Test Kit Available for
Bio-Medical Research
For More Details:
Nitrate
Test Kits (NTK)
- Standard Range (35.7 to 714 µM Nitrate/50 µL
Sample):
A. Catalog No. L-NTK-201 -- $35 (plus $5 shipping to USA Addresses)
25 Determinations using a Colorimeter in your test tubes
B.
Catalog No. L-NTK-203 -- $110 (plus $5 shipping
for USA Addresses)
100 Determinations using a Colorimeter in your test tubes
- Low Range (3.6 to 71.4 µM Nitrate/500 µL
Sample):
A. Catalog No. L-NTK-202 -- $35 (plus $5 shipping to USA Addresses)
25 Determinations using a Colorimeter in your test tubes
B. Catalog No. L-NTK-204 - $110 (plus $5 shipping
for USA Addresses)
100 Samples using a Colorimeter using your test tubes
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