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Introduction
NECi Nitrate Reductase (NaR)
Nitrate
Analysis Method has been demonstrated to match cadmium reduction for
nitrate determination in water samples using discrete analyzer technology
(Patton et al., 2006; Campbell et al., 2006a). These results “qualify”
NECi’s NaR Nitrate Analysis Method for application in most discrete
analyzers. Enzyme-based nitrate analysis overcomes the difficulties
cadmium reduction presents for discrete analyzer technology. In addition,
NECi’s Method is more environmentally benign. NECi’s new NaR, AtNaR2,
makes nitrate analysis at 37°C, easy and reliable, especially on discrete
analyzers (Campbell et al., 2006b). See NECi
PittCon2006 & NECi PittCon2007
Method
Nitrate analysis is accomplished by
reduction of nitrate to nitrite, and reaction of the resulting nitrite
with the Griess reagents to yield a highly colored compound which is
quantified using a colorimeter or spectrophotometer. In the
NECi NaR
Nitrate Analysis Method, the enzyme Nitrate Reductase (NaR) catalyzes the
reduction of nitrate to nitrite with the natural reducing agent of this
enzyme, NADH (reduced nicotinamide dinucleotide), to drive the conversion
(Campbell et al., 2006b). Since all the reagents for the NECi NaR Nitrate
Analysis Method are water soluble and stable in solution, the method is
ideally suited for discrete analyzers, which are pipet driven devices
(Campbell et al., 2006a; Patton et al., 2006).
Instruments
These discrete
analyzers are “qualified” to run the NECi NaR Nitrate Analysis Method:
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Konelab Aquakem Discrete Analyzers (see reference 3 below)
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O I Analytical DA3500 Discrete Analyzer
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New Instruments coming soon....
Other discrete
analyzers such as those sold by Astoria Pacific International, Westco Sci
Instruments, Seal Analytical, and
Systea Scientific LLC, will soon be qualified using the
method – ask your sales representative for details.
Reagents
Discrete
Autoanalyzer Reagent Kits (D-ARK) are available for specific instruments.
References
1. Campbell,
Wilbur H., Ellen R. Campbell, Lynn Egan (2006a) Green Chemistry Nitrate
Determination: An Alternative Nitrate Analysis Method. American
Laboratory, February, 2006.
http://www.americanlaboratory.com/
2. Campbell, Wilbur
H., P Song, GG Barbier (2006b) Nitrate Reductase for Nitrate Analysis in
Water. Environmental Chemistry Letters, 4: 69-73.
Download PDF.
3.
Patton, Charles J., et al. (2006) Discrete Analyzer Nitrate Analysis with
Nitrate Reductase. See:
http://pubs.acs.org/subscribe/journals/esthag-a/40/i03/html/020106news4.html
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